HDG11 Rabbit Polyclonal Antibody
cat.: ER1901-56
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Arabidopsis thaliana |
| Applications: | WB, IHC-P, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 79 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within thaliana HDG11 aa 631-680 / 722. |
| Positive control: | HDG11 recombinant protein, Arabidopsis thaliana tissue. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P ELISA |
1:500-1:2,000 1:50-1:200 1:250-1:256,000 |
| Uniprot #: | SwissProt: Q9FX31 Arabidopsis thaliana |
| Alternative names: | Homeobox-leucine zipper protein HDG11 HD-ZIP protein HDG11 Homeodomain GLABRA 2-like protein 11 Homeodomain transcription factor HDG11 Protein ENHANCED DROUGHT TOLERANCE 1 Protein HOMEODOMAIN GLABROUS 11 HDG11 EDT1 HDGL2-11 At1g73360 T9L24.43 |
Images
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Fig1:
Western blot analysis of HDG11 on Arabidopsis thaliana lysate with Rabbit anti-HDG11 antibody (ER1901-56) at 1/2,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 46 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1901-56, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 79 kDa Observed band size: 75 kDa |
|
Fig2: Immunohistochemical analysis of paraffin-embedded Arabidopsis thaliana tissue using anti-HDG11 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.