Anti-HDG11 antibody
cat.: ER1901-56
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Arabidopsis thaliana
Applications: WB, IHC-P, ELISA
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 79 kDa
Isotype: IgG
Immunogen: Synthetic peptide within A. thaliana HDG11 aa 620-690.
Positive control: A. thaliana tissue.
Subcellular location: Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  ELISA

1:500-1:2,000
1:50-1:200
1:250-1:256,000
Uniprot #: SwissProt: Q9FX31 ARATH
Alternative names: Homeobox-leucine zipper protein HDG11 HD-ZIP protein HDG11 Homeodomain GLABRA 2-like protein 11 Homeodomain transcription factor HDG11 Protein ENHANCED DROUGHT TOLERANCE 1 Protein HOMEODOMAIN GLABROUS 11 HDG11 EDT1 HDGL2-11 At1g73360 T9L24.43
Images
ER1901-56_1.jpg Fig1: Western blot analysis of HDG11 on HDG11 recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-56) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HDG11 recombinant protein with primary antibody at 1:200 dilutions
Lane 2: HDG11 recombinant protein with primary antibody at 1:1,000 dilutions
ER1901-56_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded A. thaliana
tissue using anti-HDG11 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-56, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.