GABRA1 Rabbit Polyclonal Antibody
cat.: ER1901-59
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 52 kDa
Isotype: IgG
Immunogen: Synthetic peptide within rat GABRA1 aa 11-60 / 455.
Positive control: Rat cerebellum tissue lysates, LOVO, rat brain tissue, Rat cerebellum tissue, SH-SY5Y.
Subcellular location: Postsynaptic cell membrane, cell membrane, cytoplasmic vesicle membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:50-1:100
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P14867 Human | P62813 Rat
Alternative names: ECA4 EIEE19 EJM EJM5 Gaba receptor alpha 1 polypeptide GABA(A) receptor GABA(A) receptor subunit alpha 1 GABA(A) receptor subunit alpha-1 GABA(A) receptor, alpha 1 GABRA 1 GABRA1 Gamma aminobutyric acid (GABA) A receptor alpha 1 Gamma aminobutyric acid A receptor alpha 1 Gamma aminobutyric acid receptor subunit alpha 1 Gamma aminobutyric acid type A receptor alpha1 subunit Gamma-aminobutyric acid receptor subunit alpha-1 GBRA1_HUMAN
Images
ER1901-59_1.jpg Fig1: Western blot analysis of GABRA1 on rat cerebellum tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-59, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-59_2.jpg Fig2: ICC staining of GABRA1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-59, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1901-59_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GABRA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-59_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue using anti-GABRA1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-59, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-59_5.jpg Fig5: Flow cytometric analysis of GABRA1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-59, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.