CD133 Rabbit Polyclonal Antibody
cat.: ER1901-63
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 97 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal residues of human CD133.
Positive control: NCCIT cell lysate, HT-29 cell lysate, Human kidney tissue lysate.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  FC

1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: O43490 Human
Alternative names: AC133 Antigen AC133 CD133 CORD12 Hematopoietic stem cell antigen hProminin MCDR2 MSTP061 OTTHUMP00000217744 OTTHUMP00000217745 OTTHUMP00000217746 PROM1 PROM1_HUMAN Prominin I Prominin like 1 Prominin like protein 1 precursor Prominin mouse like 1 Prominin-1 Prominin-like protein 1 Prominin1 PROML1 RP41 STGD4
Images
ER1901-63_1.jpg Fig1: Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (ER1901-63) at 1/1,000 dilution.

Lane 1: NCCIT cell lysate (10 µg/Lane)
Lane 2: HT-29 cell lysate (10 µg/Lane)
Lane 3: Human kidney tissue lysate (20 µg/Lane)

Predicted band size: 97 kDa
Observed band size: 120 kDa

Exposure time: 1 minute; ECL: K1802;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-63) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.