Anti-CD133 antibody
cat.: ER1901-63
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml
Purification: Peptide affinity purified.
Molecular weight: Predicted band size 100 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal residues of human CD133.
Positive control: SW620, HT-29 cell lysates, SW480 cell lysates, human kidney tissue, F9.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  ICC
  FC

1:500-1:2000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: O43490 Human
Alternative names: AC133 antibody Antigen AC133 antibody CD133 antibody CORD12 antibody Hematopoietic stem cell antigen antibody hProminin antibody MCDR2 antibody MSTP061 antibody OTTHUMP00000217744 antibody OTTHUMP00000217745 antibody OTTHUMP00000217746 antibody PROM1 antibody PROM1_HUMAN antibody Prominin I antibody Prominin like 1 antibody Prominin like protein 1 precursor antibody Prominin mouse like 1 antibody Prominin-1 antibody Prominin-like protein 1 antibody Prominin1 antibody PROML1 antibody RP41 antibody STGD4 antibody
Images
ER1901-63_1.jpg Fig1: Western blot analysis of CD133 on SW480 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-63_2.jpg Fig2: Western blot analysis of CD133 on HT-29 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-63, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-63_3.jpg Fig3: ICC staining of CD133 in SW620 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-63, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-63_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD133 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-63, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-63_5.jpg Fig5: Flow cytometric analysis of CD133 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-63, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.