Product Type: | Rabbit polyclonal IgG, primary antibodies |
---|---|
Species reactivity: | Human |
Applications: | WB, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | Predicted band size: 97 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within C-terminal residues of human CD133. |
Positive control: | NCCIT cell lysate, HT-29 cell lysate, Human kidney tissue lysate. |
Subcellular location: | Cell membrane. |
Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
Uniprot #: | SwissProt: O43490 Human |
Alternative names: | AC133 Antigen AC133 CD133 CORD12 Hematopoietic stem cell antigen hProminin MCDR2 MSTP061 OTTHUMP00000217744 OTTHUMP00000217745 OTTHUMP00000217746 PROM1 PROM1_HUMAN Prominin I Prominin like 1 Prominin like protein 1 precursor Prominin mouse like 1 Prominin-1 Prominin-like protein 1 Prominin1 PROML1 RP41 STGD4 |
![]() |
Fig1:
Western blot analysis of CD133 on different lysates with Rabbit anti-CD133 antibody (ER1901-63) at 1/1,000 dilution. Lane 1: NCCIT cell lysate (10 µg/Lane) Lane 2: HT-29 cell lysate (10 µg/Lane) Lane 3: Human kidney tissue lysate (20 µg/Lane) Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 1 minute; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-63) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |