ZHX2 Rabbit Polyclonal Antibody
cat.: ER1901-64
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 92 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human ZHX2 aa 538-733 / 837. |
| Positive control: | Daudi cell lysates, human esophagus tissue, mouse kidney tissue, Siha. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB IHC-P |
1:500-1:2,000 1:50-1:200 |
| Uniprot #: | SwissProt: Q9Y6X8 Human |
| Alternative names: | AFP regulator 1 AFR1 Alpha fetoprotein regulator 1 Alpha-fetoprotein regulator 1 KIAA0854 RAF Regulator of AFP Transcription factor ZHX2 ZHX2 ZHX2_HUMAN Zinc finger and homeodomain protein 2 Zinc fingers and homeoboxes 2 Zinc fingers and homeoboxes protein 2 |
Images
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Fig1:
Western blot analysis of ZHX2 on Jurkat (Human T-lymphoblastic cells) lysates with Rabbit anti-ZHX2 antibody (ER1901-64) at 1/2,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 12 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1901-64, 1/2,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 92 kDa Observed band size: 92 kDa |
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Fig2: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-ZHX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ZHX2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-64, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of Zinc fingers and homeoboxes protein 2 was done on Siha cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-64, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.