USP21 Rabbit Polyclonal Antibody
cat.: ER1901-67
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 63 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human USP21 aa 303-453 / 565. |
| Positive control: | SHSY5Y cell lysates, Rat kidney tissue, human fetal skeletal muscle tissue, Mouse heart tissue, SHSY5Y. |
| Subcellular location: | Cytoplasm. Nucleus. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:2000 1:50-1;200 1:50-1;200 |
| Uniprot #: | SwissProt: Q9UK80 Human | Q9QZL6 Mouse | B2GUX4 Rat |
| Alternative names: | Deubiquitinating enzyme 21 deubiquitinating enzyme 23 EC 3.1.2.15 ESTM28 MGC3394 NEDD8-specific protease Ubiquitin carboxyl terminal hydrolase 21 ubiquitin carboxyl terminal hydrolase 23 Ubiquitin carboxyl-terminal hydrolase 21 ubiquitin specific peptidase 21 Ubiquitin specific processing protease 21 ubiquitin specific protease 16 ubiquitin specific protease 21 ubiquitin specific protease 23 Ubiquitin thioesterase 21 Ubiquitin thiolesterase 21 ubiquitin thiolesterase 23 ubiquitin-specific protease 23, formerly Ubiquitin-specific-processing protease 21 UBP21_HUMAN USP16 v Usp21 USP23 USP23, formerly W53272 |
Images
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Fig1: Western blot analysis of USP21 on SHSY5Y cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-67, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded Rat kidney tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-67, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-67, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using anti-USP21 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-67, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig5: Flow cytometric analysis of USP21 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-67, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.