Fascin Rabbit Polyclonal Antibody
cat.: ER1901-71
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 54 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Fascin aa 1-140 / 493.
Positive control: SH-SY5Y, rat brain tissue, human tonsil tissue, human kidney tissue, mouse testis tissue.
Subcellular location: Cytoskeleton. Cytosol.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q16658 Human | Q61553 Mouse | P85845 Rat
Alternative names: 55 kDa actin bundling protein 55 kDa actin-bundling protein Actin bundling protein actin bundling protein, 55-KD FAN 1 FAN1 Fascin 1 Fascin actin bundling protein 1 Fascin Fascin homolog 1 actin bundling protein (Strongylocentrotus purpuratus) Fascin homolog 1 Fascin, sea urchin, homolog of, 1 Fascin1 FLJ38511 FSCN 1 FSCN1 FSCN1_HUMAN HSN p55 Singed (Drosophila) like (sea urchin fascin homolog like) Singed drosophila homolog like Singed like (fascin homolog sea urchin) (Drosophila) Singed like (fascin homolog sea urchin) Singed like protein Singed, drosophila, homolog of Singed-like protein SNL Strongylocentrotus purpuratus
Images
ER1901-71_1.jpg Fig1: Western blot analysis of Fascin on different lysates with Rabbit anti-Fascin antibody (ER1901-71) at 1/1,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Fascin KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 54 kDa
Observed band size: 54 kDa

Exposure time: 40 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-71) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1901-71_2.jpg Fig2: Western blot analysis of Fascin on SH-SY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:1,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:50,000 dilution was used for 1 hour at room temperature.
ER1901-71_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1901-71_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1901-71_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1901-71_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Fascin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-71) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
ER1901-71_7.jpg Fig7: Flow cytometric analysis of Fascin was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-71, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.