Alpha-dystroglycan Rabbit Polyclonal Antibody
cat.: ER1901-72
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 97 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human Alpha-dystroglycan aa 15-64 / 895.
Positive control: Neuro-2a cell lysate, C6 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, SkBr3, Rat skeletal muscle tissue, human placenta tissue, Mouse heart tissue, A431.
Subcellular location: Secreted, extracellular space; Cell membrane, Cytoplasm, cytoskeleton, Nucleus, nucleoplasm, sarcolemma, Postsynaptic cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:5,000
1:50-1:200
1:50-1:200
1:50-1:200
Uniprot #: SwissProt: Q14118 Human | Q62165 Mouse
Entrez Gene: 114489 Rat
Alternative names: 156DAG A3a Agrin receptor AGRNR Alpha-DG alpha-DG-N Beta-DG Beta-dystroglycan DAG Dag1 DAG1_HUMAN Dystroglycan 1 (dystrophin-associated glycoprotein 1) Dystroglycan Dystroglycan, alpha Dystrophin-associated glycoprotein 1 MDDGC7 MDDGC9 OTTHUMP00000210857 OTTHUMP00000210858
Images
ER1901-72_1.jpg Fig1: Western blot analysis of Alpha-dystroglycan on different lysates with Rabbit anti-Alpha-dystroglycan antibody (ER1901-72) at 1/5,000 dilution.

Lane 1: Neuro-2a cell lysate (20 µg/Lane)
Lane 2: C6 cell lysate (20 µg/Lane)
Lane 3: Mouse heart tissue lysate (40 µg/Lane)
Lane 4: Rat heart tissue lysate (40 µg/Lane)

Predicted band size: 97 kDa
Observed band size: 97 kDa

Exposure time: 10 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-72) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1901-72_2.jpg Fig2: ICC staining of Alpha-dystroglycan in SkBr3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-72, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-72_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue using anti-Alpha-dystroglycan antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-72, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-72_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Alpha-dystroglycan antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-72, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-72_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded Mouse heart tissue using anti-Alpha-dystroglycan antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-72, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-72_6.jpg Fig6: Flow cytometric analysis of Alpha-dystroglycan was done on A431 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-72, 1/100) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.