GABARAPL2 Rabbit Polyclonal Antibody
cat.: ER1901-73
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Protein A affinity purified.
Molecular weight: 14 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human GABARAPL2 aa 1-117 / 117.
Positive control: 293 cell lysate, SHSY5Y cell lysate, SHSY5Y, Siha, Rat cerebellum tissue, Human prostate tissue, Mouse small intestine tissue.
Subcellular location: Cytoplasmic vesicle. Golgi apparatus.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:500-1:2000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P60520 Human | P60521 Mouse | P60522 Rat
Alternative names: ATG8 ATG8C FLC3A GABA(A) receptor-associated protein-like 2 Gabarapl2 Gamma-aminobutyric acid receptor-associated protein-like 2 Ganglioside expression factor 2 GATE-16 GATE16 GBRL2_HUMAN GEF-2 GEF2 General protein transport factor p16 Golgi-associated ATPase enhancer of 16 kDa MAP1 light chain 3-related protein
Images
ER1901-73_1.jpg Fig1: Western blot analysis of GABARAPL2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-73, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: SHSY5Y cell lysate
ER1901-73_2.jpg Fig2: ICC staining of GABARAPL2 in SHSY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-73, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-73_3.jpg Fig3: ICC staining of GABARAPL2 in Siha cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-73, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-73_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue using anti-GABARAPL2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-73, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-73_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded Human prostate tissue using anti-GABARAPL2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-73, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-73_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded Mouse small intestine tissue using anti-GABARAPL2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-73, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-73_7.jpg Fig7: Flow cytometric analysis of GABARAPL2 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-73, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.