BCL-6 Rabbit Polyclonal Antibody
cat.: ER1901-74
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 79 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human BCL-6 aa 287-464 / 706.
Positive control: Raji cell lysates, HepG2, Jurkat.
Subcellular location: Nucleus.
Recommended Dilutions:

Uniprot #: SwissProt: P41182 Human
Alternative names: B cell CLL/lymphoma 6 B cell lymphoma 6 protein B-cell lymphoma 5 protein B-cell lymphoma 6 protein BCL 5 Bcl 6 BCL-5 BCL-6 BCL5 BCL6 BCL6_HUMAN BCL6A cys his2 zinc finger transcription factor cys-his2 zinc finger transcription factor LAZ 3 LAZ 3 protein LAZ3 Lymphoma Associated Zinc Finger Gene On Chromosome 3 (LAZ3) Lymphoma associated zinc finger gene on chromosome 3 Protein LAZ-3 ZBTB 27 ZBTB27 Zinc finger and BTB domain containing protein 27 Zinc finger and BTB domain-containing protein 27 (ZBTB27) Zinc finger and BTB domain-containing protein 27 Zinc Finger Protein 51 (ZNF51) Zinc finger protein 51 zinc finger transcription factor BCL6S ZNF 51 ZNF51
ER1901-74_1.jpg Fig1: Western blot analysis of BCL-6 on Raji cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-74, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-74_2.jpg Fig2: ICC staining of BCL-6 in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-74, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-74_3.jpg Fig3: Flow cytometric analysis of BCL-6 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-74, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).