p15 INK4b Rabbit Polyclonal Antibody
cat.: ER1901-75
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF-Cell, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 15 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human p15 INK4b aa 1-138 / 138. |
| Positive control: | Hela, SiHa, rat kidney tissue, human esophagus cancer tissue, human small intestine tissue, mouse liver tissue. |
| Subcellular location: | Cytoplasm. Also found in the nucleus. |
| Recommended Dilutions:
WB IF-Cell IHC-P FC |
1:500 1:50-1:200 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P42772 Human | P55271 Mouse | P55272 Rat |
| Alternative names: | CDK inhibitory protein CDK4B Inhibitor Cdkn2b CDN2B_HUMAN Cyclin dependent kinase 4 inhibitor B Cyclin Dependent Kinase Inhibitor 2B Cyclin dependent kinase inhibitor 2B p15 inhibits CDK4 Cyclin dependent kinases 4 and 6 binding protein Cyclin-dependent kinase 4 inhibitor B INK4B MTS 2 MTS-2 MTS2 Multiple tumor suppressor 2 Multiple Tumor Supressor 2 OTTHUMP00000021154 OTTHUMP00000021155 p14 CDK inhibitor p14 INK4b p14-INK4b P15 p15 CDK inhibitor p15 inhibits CDK4 p15 INK4b p15-INK4b p15INK4B TP 15 TP15 |
Images
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Fig1: Western blot analysis of p15 INK4b on recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-75, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: ICC staining p15 INK4b in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1901-75) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: ICC staining p15 INK4b in SiHa cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (ER1901-75) at a dilution of 1:50 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-p15 INK4b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-75) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig5: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-p15 INK4b antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-75) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX. |
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Fig6: Flow cytometric analysis of p15 INK4b was done on Hela cells. The cells were fixed, permeabilized and stained with p15 INK4b antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-Rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.