Desmoglein 3 Rabbit Polyclonal Antibody
cat.: ER1901-81
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size 108 kDa.
Isotype: IgG
Immunogen: Recombinant protein within Human DSG3 aa 709-897 / 999.
Positive control: A431 cell lysates, PC-3M, rat esophagus tissue, human esophagus cancer tissue, mouse skin tissue, Hela.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:500-1:2,000
1:100-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P32926 Human | O35902 Mouse | D3ZL74 Rat
Alternative names: 130 kD pemphigus vulgaris antigen 130 kDa pemphigus vulgaris antigen Balding Cadherin family member 6 CDHF 6 CDHF6 Desmoglein 3 Desmoglein 3 precursor Desmoglein-3 Desmoglein3 DKFZp686P23184 DSG 3 DSG3 DSG3_HUMAN Pemphigus vulgaris antigen PVA
Images
ER1901-81_1.png Fig1: Western blot analysis of Desmoglein 3 on A431 cell lysates with Rabbit anti-Desmoglein 3 antibody (ER1901-81) at 1/500 dilution.

Lysates/proteins at 10 µg/Lane.

Predicted band size: 108 kDa
Observed band size: 130 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-81) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER1901-81_2.png Fig2: Immunocytochemistry analysis of PC-3M cells labeling Desmoglein 3 with Rabbit anti-Desmoglein 3 antibody (ER1901-81) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-Desmoglein 3 antibody (ER1901-81) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

β-tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 647, HA1127) were used as the secondary antibody at 1/1,000 dilution.
ER1901-81_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat esophagus tissue using anti-DSG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-81) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1901-81_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue using anti-DSG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-81) at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1901-81_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-DSG3 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody (ER1901-81) at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
ER1901-81_6.jpg Fig6: Flow cytometric analysis of DSG3 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-81, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; blue).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.