CD35 Rabbit Polyclonal Antibody
cat.: ER1901-82
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 224 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human CD35 aa 290-339 / 2,039. |
| Positive control: | K562 cell, 293T cell, HL-60 cell. |
| Subcellular location: | Membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:2,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P17927 Human |
| Alternative names: | C3 binding protein C3b/C4b receptor C3BR C4BR CD 35 CD35 CD35 antigen complement component (3b/4b) receptor 1 (Knops blood group) complement component (3b/4b) receptor 1 including Knops blood group system Complement component receptor 1 Complement receptor 1 Complement receptor type 1 CR 1 CR1 CR1_HUMAN KN Knops blood group antigen |
Images
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Fig1:
Western blot analysis of CD35 on K562 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-82, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: K562 cell lysate Lane 2: K562 cell lysate, the primary antibody was preincubated with immunization peptide. |
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Fig2: ICC staining of CD35 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-82, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue). |
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Fig3: Flow cytometric analysis of CD35 was done on HL-60 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-82, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.