B3GAT1 Rabbit Polyclonal Antibody
cat.: ER1901-83
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 38 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within Human B3GAT1 aa 285-334 / 334.
Positive control: 293 cell lysates, F9, SHSY5Y, Rat brain tissue, mouse brain tissue.
Subcellular location: Golgi apparatus membrane, Secreted, extracellular region.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
1:1,000
1:100
1:200
Uniprot #: SwissProt: Q9P2W7 Human | Q9CW73 Mouse | O35789 Rat
Alternative names: 3-glucuronyltransferase 1 3-glucuronyltransferase B3GA1_HUMAN B3gat1 Beta 1 3 glucuronyltransferase 1 Beta-1 Beta-1,3-glucuronyltransferase 1 CD 57 CD57 CD57 antigen Galactosylgalactosylxylosylprotein 3 beta glucuronosyltransferase 1 Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1 GlcAT P GlcAT-P GLCATP GlcUAT P GlcUAT-P GlcUATP Glucuronosyltransferase P HNK 1 HNK1 LEU 7 LEU7 LEU7 antigen NK 1 NK1 UDP GlcUA glycoprotein beta 1 3 glucuronyltransferase UDP-GlcUA:glycoprotein beta-1
Images
ER1901-83_1.jpg Fig1: Western blot analysis of B3GAT1 on 293 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-83, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Predicted band size: 38 kDa
Observed band size: 45 kDa(Glycosylation)
ER1901-83_2.jpg Fig2: ICC staining of B3GAT1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-83, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-83_3.jpg Fig3: ICC staining of B3GAT1 in SHSY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 10% negative goat serum for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-83, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-83_4.png Fig4: Immunocytochemistry analysis of Hela cells labeling B3GAT1 with Rabbit anti-B3GAT1 antibody (ER1901-83) at 1/200 dilution.

Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-B3GAT1 antibody (ER1901-83) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. Nuclear DNA was labelled in blue with DAPI.

β-tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 674, HA1127) were used as the secondary antibody at 1/1,000 dilution.
ER1901-83_5.png Fig5: Immunohistochemical analysis of paraffin-embedded Rat brain tissue using anti-B3GAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-83, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-83_6.png Fig6: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-B3GAT1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-83, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-83_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human brain tissue with Rabbit anti-B3GAT1 antibody (ER1901-83) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-83) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.