CNGA4 Rabbit Polyclonal Antibody
cat.: ER1901-88
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size 65 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within rat CNGA4 aa 1-50 / 575.
Positive control: A549 cell lysate, A431 cell lysate, human brain tissue lysate, SHSY5Y cell lysate, N2A, SHSY5Y, Hela.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  ICC
  FC

1:500-1:1000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q8IV77 Human | Q3UW12 Mouse | Q64359 Rat
Alternative names: Cyclic nucleotide-gated cation channel alpha-4 Cyclic nucleotide-gated channel alpha-4 CNG channel alpha-4 CNG-4 CNG4 Cnga4 Cyclic nucleotide-gated olfactory channel subunit OCNC2 CNG4
Images
ER1901-88_1.jpg Fig1: Western blot analysis of CNGA4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: A549 cell lysate
Lane 2: A431 cell lysate
ER1901-88_2.jpg Fig2: Western blot analysis of CNGA4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-88, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human brain tissue lysate
Lane 2: SHSY5Y cell lysate
ER1901-88_3.jpg Fig3: ICC staining of CNGA4 in N2A cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-88, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-88_4.jpg Fig4: ICC staining of CNGA4 in SHSY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-88, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-88_5.jpg Fig5: Flow cytometric analysis of CNGA4 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-88, 1/100) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; purple).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.