CACNG2 Rabbit Polyclonal Antibody
cat.: ER1901-89
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Immunogen affinity purified.
Molecular weight: 36 kDa (Predicted band size)
Isotype: IgG
Immunogen: Synthetic peptide within Human CACNG2 aa 196-245 / 323.
Positive control: Mouse cerebellum tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, A549, SHSY5Y, mouse brain tissue, rat Cerebellum tissue.
Subcellular location: Cell junction, Membrane.
Recommended Dilutions:
  WB
  IHC-P
  ICC
  FC

1:500-1:2000
1;50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9Y698 Human | O88602 Mouse | Q71RJ2 Rat
Alternative names: AW060990 B230105C07Rik B930041E13Rik Cacng2 Calcium channel voltage dependent gamma subunit 2 CaV gamma 2 CCG2_HUMAN Ipr328 MGC123981 MGC138502 MGC138504 Neuronal voltage gated calcium channel gamma 2 subunit Neuronal voltage-gated calcium channel gamma-2 subunit Stargazer stg TARP gamma-2 Transmembrane AMPAR regulatory protein gamma-2 Voltage dependent calcium channel gamma 2 subunit Voltage-dependent calcium channel gamma-2 subunit Wag Waggler
Images
ER1901-89_1.jpg Fig1: Western blot analysis of CACNG2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-89, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse cerebellum tissue lysate
Lane 2: Mouse brain tissue lysate
Lane 3: Rat brain tissue lysate
Predicted band size: 36 kDa
Observed band size: 41 kDa
ER1901-89_2.jpg Fig2: ICC staining of CACNG2 in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-89, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-89_3.jpg Fig3: ICC staining of CACNG2 in SHSY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-89, 1/50) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-89_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CACNG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-89, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-89_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat Cerebellum tissue using anti-CACNG2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-89, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-89_6.jpg Fig6: Flow cytometric analysis of CACNG2 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-89, 1/100) (purple). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; yellow).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.