PKM2 Rabbit Polyclonal Antibody
cat.: ER1901-90
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human PKM aa 380-450. |
| Positive control: | SiHa cell lysate, mouse spleen tissue lysate, MDA-MB-231 whole cell lysate, F9. |
| Subcellular location: | Nucleus, cytoplasm. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: P14618 Human | P52480 Mouse |
| Alternative names: | CTHBP Cytosolic thyroid hormone-binding protein KPYM_HUMAN OIP-3 Opa-interacting protein 3 p58 pkm PKM1 PKM2 Pyruvate kinase 2/3 Pyruvate kinase muscle isozyme Pyruvate kinase PKM THBP1 Thyroid hormone-binding protein 1 Tumor M2-PK |
Images
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Fig1:
Western blot analysis of PKM2 on different lysates with Rabbit anti-PKM2 antibody (ER1901-90) at 1/1,000 dilution. Lane 1: SiHa cell lysate (15 µg/Lane) Lane 2: Mouse spleen tissue lysate (30 µg/Lane) Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 8 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-90) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. |
|
Fig2:
All lanes: Western blot analysis of PKM with anti-PKM antibody (ER1901-90) at 1:500 dilution. Lane 1: Wild-type MDA-MB-231 whole cell lysate. Lane 2: PKM knockout MDA-MB-231 whole cell lysate. ER1901-90 was shown to specifically react with PKM in wild-type MDA-MB-231 cells. No band was observed when PKM knockout samples were tested. Wild-type and PKM knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary Anti-PKM antibody (ER1901-90, 1/500) and Anti-GAPDH antibody (ET1601-4, 1/10000) were used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG H&L (HRP) Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. Cell lysate was provided by Ubigene Biosciences (Ubigene Biosciences Co., Ltd., Guangzhou, China). |
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Fig3: Flow cytometric analysis of PKM2 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-90, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.