delta 1 Catenin/CAS Rabbit Polyclonal Antibody
cat.: ER1901-92
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IF-Cell, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size 108 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within Human delta 1 Catenin / CAS aa 80-129 / 968.
Positive control: HUVEC cell lysates, mouse stomach tissue lysates, rat skin tissue lysates, A431, Siha, rat testis tissue, human breast cancer tissue, human kidney tissue, mouse testis tissue.
Subcellular location: Cell membrane, Cytoplasm, Membrane, Nucleus.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
  FC
1:1,000-1:2000
1:100-1:500
1:100-1:500
1:50-1:200
Uniprot #: SwissProt: O60716 Human | P30999 Mouse | D3ZZZ9 Rat
Alternative names: Cadherin associated Src substrate Cadherin-associated Src substrate CAS Catenin (cadherin associated protein) delta 1 Catenin delta 1 Catenin delta Catenin delta-1 CTND1_HUMAN CTNND 1 CTNND CTNND1 delta 1 Catenin KIAA0384 p120 P120 CAS p120 catenin P120 CTN p120(cas) p120(ctn) P120CAS P120CTN
Images
ER1901-92_1.jpg Fig1: Western blot analysis of delta 1 Catenin/CAS on different lysates with Rabbit anti-delta 1 Catenin/CAS antibody (ER1901-92) at 1/2,000 dilution.

Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-delta 1 Catenin/CAS KD cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 108 kDa
Observed band size: 70-120 kDa

Exposure time: 16 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1901-92) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1901-92_2.jpg Fig2: Western blot analysis of delta 1 Catenin/CAS on HUVEC cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-92, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-92_3.jpg Fig3: Western blot analysis of delta 1 Catenin/CAS on mouse stomach tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-92, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-92_4.jpg Fig4: ICC staining of delta 1 Catenin/CAS in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-92, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-92_5.jpg Fig5: ICC staining of delta 1 Catenin/CAS in Siha cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-92, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-92_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-delta 1 Catenin/CAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-92, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-92_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-delta 1 Catenin/CAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-92, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-92_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-delta 1 Catenin/CAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-92, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-92_9.jpg Fig9: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-delta 1 Catenin/CAS antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-92, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-92_10.jpg Fig10: Flow cytometric analysis of delta 1 Catenin/CAS was done on Siha cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-92, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.