SLC8B1 Rabbit Polyclonal Antibody
cat.: ER1901-93
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 64 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human SLC8B1 aa 229-406 / 584.
Positive control: Mouse tonsil tissue lysates, SHSY5Y, SKOV-3, rat kidney tissue, human tonsil tissue, human pancreas tissue, Siha.
Subcellular location: Membrane, Mitochondrion, Mitochondrion inner membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:500-1:1000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q6J4K2 Human | Q925Q3 Mouse | Q6AXS0 Rat
Alternative names: FLJ22233 Na(+)/K(+)/Ca(2+) exchange protein 6 Na(+)/K(+)/Ca(2+)-exchange protein 6 NCKX6 NCKX6_HUMAN NCLX Slc24a6 Sodium/potassium/calcium exchanger Sodium/potassium/calcium exchanger 6 Solute carrier family 24 (sodium/potassium/calcium exchanger) member 6 Solute carrier family 24 member 6
Images
ER1901-93_1.jpg Fig1: Western blot analysis of SLC8B1 on mouse tonsil tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-93, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-93_2.jpg Fig2: ICC staining of SLC8B1 in SHSY5Y cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-93, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-93_3.jpg Fig3: ICC staining of SLC8B1 in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-93, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-93_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-SLC8B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-93, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-93_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-SLC8B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-93, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-93_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-SLC8B1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-93, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-93_7.jpg Fig7: Flow cytometric analysis of SLC8B1 was done on Siha cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-93, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.