FOLR1 Rabbit Polyclonal Antibody
cat.: ER1901-95
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size 30 kDa.
Isotype: IgG
Immunogen: Recombinant protein within Human FOLR1 aa 42-257 / 257.
Positive control: SKBR3 cell lysate, human placenta tissue lysate, human lung cancer tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue.
Subcellular location: Cell membrane, Cytoplasmic vesicle, Endosome, Membrane, Secreted.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P15328 Human | P35846 Mouse | G3V8M6 Rat
Alternative names: adult Adult folate binding protein Adult folate-binding protein FBP Folate Binding Protein Folate Receptor 1 Adult Folate receptor 1 Folate Receptor 1 Precursor Folate receptor adult Folate receptor alpha Folate receptor FOLR FOLR1 FOLR1_HUMAN FR alpha FR-alpha FRalpha KB cells FBP MOV18 Ovarian cancer associated antigen Ovarian tumor associated antigen Ovarian tumor associated antigen MOv18 Ovarian tumor-associated antigen MOv18
Images
ER1901-95_1.jpg Fig1: Western blot analysis of FOLR1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-95, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: SKBR3 cell lysate
Lane 2: human placenta tissue lysate
ER1901-95_2.png Fig2: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-FOLR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-95, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-95_3.png Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FOLR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-95, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-95_4.png Fig4: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-FOLR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-95, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-95_5.png Fig5: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-FOLR1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1901-95, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1901-95_6.jpg Fig6: Flow cytometric analysis of FOLR1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-95, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.