Anti-Progesterone receptor antibody
cat.: ER1901-96
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Protein affinity purified.
Molecular weight: Predicted band size 99 kDa.
Isotype: IgG
Immunogen: Recombinant protein within Human Progesterone receptor aa 30-250.
Positive control: AN3CA cell lysates, A549 cell, MCF-7 cell.
Subcellular location: Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane, Nucleus.
Recommended Dilutions:
  WB
  ICC
  FC

1:500-1:2000
1:100-1:500
1:50-1:100
Uniprot #: SwissProt: P06401 Human
Alternative names: NR3C3 antibody Nuclear receptor subfamily 3 group C member 3 antibody PGR antibody PR antibody PRA antibody PRB antibody PRGR_HUMAN antibody Progesterone receptor antibody Progestin receptor form A antibody Progestin receptor form B antibody
Images
ER1901-96_1.jpg Fig1: Western blot analysis of Progesterone receptor on AN3CA cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1901-96, 1/100) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1901-96_2.jpg Fig2: ICC staining of Progesterone receptor in A549 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-96, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-96_3.jpg Fig3: ICC staining of Progesterone receptor in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1901-96, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1901-96_4.jpg Fig4: Flow cytometric analysis of Progesterone receptor was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1901-96, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.