CD45 Rabbit Polyclonal Antibody
cat.: ER1902-02
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 147 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD45 aa 1-60. |
| Positive control: | U937 cell lysates, Jurkat cell lysates, Jurkat. |
| Subcellular location: | Cell membrane, membrane raft. |
| Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
| Uniprot #: | SwissProt: P08575 Human |
| Alternative names: | B220 CD 45 CD45 CD45 antigen CD45R GP180 L-CA LCA Leukocyte common antigen loc Ly-5 LY5 Ly5, homolog of Lyt-4 OTTHUMP00000033813 OTTHUMP00000033816 OTTHUMP00000033817 OTTHUMP00000038574 Protein tyrosine phosphatase receptor type c polypeptide Protein tyrosine phosphatase, receptor type C protein tyrosine phosphatase, receptor type, C Protein tyrosine phosphatase, receptor type, c polypeptide Ptprc PTPRC_HUMAN Receptor-type tyrosine-protein phosphatase C T200 T200 glycoprotein T200 leukocyte common antigen |
Images
|
Fig1:
Western blot analysis of CD45 on U937 cell lysates using anti-CD45 antibody. Lane 1: Anti-CD45 Antibody (1/500). Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide. |
|
Fig2:
Western blot analysis of CD45 on Jurkat cell lysates using anti-CD45 antibody. Lane 1: Anti-CD45 Antibody (1/500). Lane 2: Anti-CD45 Antibody, pre-incubated with the immunizaiton peptide. |
|
Fig3: Flow cytometric analysis of CD45 was done on Jurkat cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-02, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.