ER1902-07

DPP6 Rabbit Polyclonal Antibody

cat.: ER1902-07

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Cell, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size 98 kDa
Isotype:	IgG
Immunogen:	Synthetic peptide within Human DPP6 aa 402-451 / 865.
Positive control:	Rat brain tissue lysate, mouse brain tissue lysate, F9, SH-SY5Y, rat brain tissue, mouse brain tissue, SHSY5Y.
Subcellular location:	Cell membrane.
Recommended Dilutions: WB IHC-P IF-Cell FC	1:500-1:2000 1:50-1:200 1:50-1:200 1:50-1:100
Uniprot #:	SwissProt: P42658 Human \| Q9Z218 Mouse \| P46101 Rat
Alternative names:	Dipeptidyl aminopeptidase IV related protein Dipeptidyl aminopeptidase like protein 6 Dipeptidyl aminopeptidase related protein Dipeptidyl peptidase 6 Dipeptidyl peptidase IV like protein Dipeptidyl peptidase IV related protein Dipeptidylpeptidase 6 Dipeptidylpeptidase VI DPP VI DPPX VF2

Images

	Fig1: Western blot analysis of DPP6 on different lysates with Rabbit anti-DPP6 antibody (ER1902-07) at 1/1,000 dilution. Lane 1: Rat brain tissue lysate Lane 2: Mouse brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 98 kDa Observed band size: 100 kDa Exposure time: 2 minutes; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-07) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
	Fig2: ICC staining of DPP6 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-07, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
	Fig3: Immunocytochemistry analysis of SH-SY5Y cells labeling DPP6 with Rabbit anti-DPP6 antibody (ER1902-07) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 ℃, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were then incubated with Rabbit anti-DPP6 antibody (ER1902-07) at 1/200 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

	Fig4: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DPP6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1902-07, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DPP6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1902-07, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Flow cytometric analysis of DPP6 was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-07, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.