CD163 Rabbit Polyclonal Antibody
cat.: ER1902-09
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 125 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human CD163 aa 1000-1100. |
| Positive control: | SHSY5Y cell lysates, HCT116, human liver tissue lysate, human placenta tissue lysate. |
| Subcellular location: | Secreted. Cell membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:1000 1:50-1:100 |
| Uniprot #: | SwissProt: Q86VB7 Human |
| Alternative names: | C163A_HUMAN CD 163 CD163 CD163 antigen CD163 molecule Hemoglobin scavenger receptor M130 M130 antigen precursor Macrophage associated antigen MM130 OTTHUMP00000238617 OTTHUMP00000238618 OTTHUMP00000238619 OTTHUMP00000238620 SCARI1 Scavenger receptor cysteine rich type 1 protein M130 sCD163 Soluble CD163 |
Images
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Fig1: Western blot analysis of CD163 on SHSY5Y cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-09, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Flow cytometric analysis of CD163 was done on HCT116 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-09, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
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Fig3:
Western blot analysis of CD163 on different lysates with Rabbit anti-CD163 antibody (ER1902-09) at 1/1,000 dilution. Lane 1: Human liver tissue lysate Lane 2: Human placenta tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 125 kDa Observed band size: 130 kDa Exposure time: 1 minute; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-09) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.