Muc1 Rabbit Polyclonal Antibody
cat.: ER1902-10
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IF-Cell, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size 122 kDa
Isotype: IgG
Immunogen: Synthetic peptide immune sequence is APDTRPAPGSTAPPAHGVTSC.
Positive control: MCF-7 cell lysate, Hela cell lysate, MCF-7, human colon tissue, human skin tissue, human breast carcinoma tissue, human kidney tissue, human uterus tissue.
Subcellular location: Plasma membrane.
Recommended Dilutions:
  WB
  IF-Cell
  IHC-P
1:500-1:2,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: P15941 Human
Alternative names: ADMCKD ADMCKD1 Breast carcinoma associated antigen DF3 Breast carcinoma-associated antigen DF3 CA 15-3 CA15 3 CA15 3 antigen CA15-3 CA15.3 Cancer antigen 15-3 Carcinoma associated mucin Carcinoma-associated mucin CD 227 CD227 DF3 antigen EMA Episialin Epithelial Membrane Antigen H23 antigen H23AG KL 6 KL-6 KL6 Krebs von den Lungen-6 MAM 6 MAM6 MCD MCKD MCKD1 Medullary cystic kidney disease 1 (autosomal dominant) Medullary cystic kidney disease, autosomal dominant MUC 1 MUC-1 MUC-1/SEC MUC-1/X MUC1 MUC1-alpha MUC1-beta MUC1-CT MUC1-NT MUC1/ZD MUC1_HUMAN Mucin 1 Mucin 1 cell surface associated Mucin 1 transmembrane Mucin 1, cell surface associated Mucin-1 subunit beta Peanut reactive urinary mucin Peanut-reactive urinary mucin PEM PEMT Polymorphic epithelial mucin PUM Tumor associated epithelial membrane antigen Tumor associated epithelial mucin Tumor associated mucin Tumor-ass......
Images
ER1902-10_1.jpg Fig1: Western blot analysis of Muc1 on different lysates with Rabbit anti-Muc1 antibody (ER1902-10) at 1/2,000 dilution.

Lane 1: MCF-7 cell lysate
Lane 2: Hela cell lysate

Lysates/proteins at 10 µg/Lane.

Predicted band size: 122 kDa
Observed band size: 200 kDa

Exposure time: 2 minutes;

6% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-10) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.
ER1902-10_2.jpg Fig2: ICC staining of Muc1 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-10, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1902-10_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Muc1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-10_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-Muc1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-10_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Muc1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-10_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Muc1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-10_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Muc1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-10, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.