Claudin 18.1 Rabbit Polyclonal Antibody
cat.: ER1902-11
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 28 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within human Claudin18 aa 28-80. |
| Positive control: | NCI-H358 (Human lung adenocarcinoma cell) cell lysates, NCI-H1299 (Human non-small cell Lung carcinoma cell) cell lysates, F9. |
| Subcellular location: | Cell membrane. |
| Recommended Dilutions:
WB FC |
1:5,000 1:500-1:1,000 |
| Uniprot #: | SwissProt: P56856 Human | P56857 Mouse |
| Alternative names: | Claudin 18 Claudin-18 CLD18_HUMAN CLDN18 SFTA5 SFTPJ |
Images
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Fig1:
Western blot analysis of Claudin 18.1 on NCI-H358 (Human lung adenocarcinoma cell) cell lysate with Rabbit anti-Claudin 18.1 antibody (ER1902-11) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 43 seconds; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1902-11, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28 kDa Observed band size: 28 kDa |
|
Fig2:
Western blot analysis of Claudin 18.1 on NCI-H1299 (Human non-small cell Lung carcinoma cell) cell lysate with Rabbit anti-Claudin 18.1 antibody (ER1902-11) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 3 minutes; ECL: K1802 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1902-11, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 28 kDa Observed band size: 28 kDa |
|
Fig3:
Flow cytometric analysis of F9 cells labeling Claudin 18.1. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (ER1902-11, 1ug/ml) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.