RIP3 Rabbit Polyclonal Antibody
cat.: ER1902-12
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Mouse, Rat |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 57 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Mouse RIP3 aa 440-486 / 486. |
| Positive control: | MEF cell lysates. |
| Subcellular location: | Mitochondrion, Cytosol, Plasma membrane. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: Q9QZL0 Mouse | Q9Z2P5 Rat |
| Alternative names: | Receptor interacting protein 3 Receptor interacting serine threonine kinase 3 Receptor interacting serine/threonine protein kinase 3 Receptor-interacting protein 3 Receptor-interacting serine/threonine-protein kinase 3 RIP 3 RIP like protein kinase 3 RIP-3 RIP-like protein kinase 3 RIPK 3 RIPK3 RIPK3_HUMAN |
Images
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Fig1:
All lanes: Western blot analysis of RIP3 with anti-RIP3 antibody (ER1902-12) at 1:1,000 dilution. Lane 1: Wild-type MEF whole cell lysate (20 µg). Lane 2: RIP3 knockout MEF whole cell lysate (20 µg). ER1902-12 was shown to specifically react with RIP3 in wild-type MEF cells. No band was observed when RIP3 knockout sample was tested. Wild-type and RIP3 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1902-12, 1/1,000) and Loading control antibody (Rabbit anti-β-actin, R1207-1, 1/1,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.