AP-4 complex subunit sigma Rabbit Polyclonal Antibody
cat.: ER1902-15
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Arabidopsis thaliana |
| Applications: | IF-Tissue, IHC-P, ELISA |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 17 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within thaliana AP-4 complex subunit sigma aa 51-100 / 143. |
| Positive control: | A. thaliana tissue. |
| Subcellular location: | Trans-Golgi network, coated pit. |
| Recommended Dilutions:
IF-Tissue IHC-P ELISA |
1:50-1:100 1:50-1:200 1:10,000-1:20,000 |
| Uniprot #: | SwissProt: O82201 ARATH |
| Alternative names: | AP-4 complex subunit sigma AP-4 adaptor complex subunit sigma Adaptor-related protein complex 4 subunit sigma Sigma subunit of AP-4 Sigma4-adaptin At2g19790 F6F22.18 |
Images
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Fig1: Immunofluorescence staining of paraffin- embedded A. thaliana using anti-AP-4 complex subunit sigma rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-15 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature. |
|
Fig2: Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-AP-4 complex subunit sigma antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-15, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.