KChIP1 Rabbit Polyclonal Antibody
cat.: ER1902-17
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Rat |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size 27 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human KChIP1 aa 1-50 / 227. |
| Positive control: | Human skin tissue lysates, rat brain tissue, SH-SY5Y. |
| Subcellular location: | Cell membrane, cytoplasm, dendrite. |
| Recommended Dilutions:
WB IHC-P FC |
1:500 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q9NZI2 Human | Q8R426 Rat |
| Alternative names: | A type potassium channel modulatory protein 1 A-type potassium channel modulatory protein 1 KChIP1 KCIP1_HUMAN Kcnip1 Kv channel interacting protein 1 Kv channel-interacting protein 1 MGC9 MGC95 Potassium channel interacting protein 1 Potassium channel-interacting protein 1 VABP Vesicle APC binding protein Vesicle APC-binding protein |
Images
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Fig1: Western blot analysis of KChIP1 on human skin tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-17, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-KChIP1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-17, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of KChIP1 was done on SH-SY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-17, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.