Beta-adaptin-like protein A Rabbit Polyclonal Antibody
cat.: ER1902-18
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Arabidopsis thaliana |
| Applications: | IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 93 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within thaliana Beta-adaptin-like protein A aa 782-831 / 841. |
| Positive control: | A. thaliana tissue. |
| Subcellular location: | Golgi apparatus, trans-Golgi network, clathrin-coated vesicle membrane. |
| Recommended Dilutions:
IHC-P FC |
1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q9LDK9 ARATH |
| Alternative names: | Beta-adaptin-like protein A At-bA-Ad At-betaA-Ad AP complex subunit beta-A Adaptor protein complex AP subunit beta-A Beta-adaptin A Clathrin assembly protein complex beta large chain A BETAA-AD At5g11490 F15N18.80 |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded A. thaliana tissue using anti-Beta-adaptin-like protein A antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-18, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Immunofluorescence staining of paraffin- embedded A. thaliana using anti-Beta-adaptin-like protein A antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-18 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature. |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.