DLL4 Rabbit Polyclonal Antibody
cat.: ER1902-20
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 75 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide corresponding to N terminal Human DLL4. |
| Positive control: | 293 cell lysate, mouse placenta tissue lysate, 293T. |
| Subcellular location: | Cell membrane, Membrane. |
| Recommended Dilutions:
WB FC |
1:500-1:2,000 1:50-1:100 |
| Uniprot #: | SwissProt: Q9NR61 Human | Q9JI71 Mouse |
| Alternative names: | AOS6 Delta 4 delta 4 precursor Delta ligand 4 delta ligand 4 precursor Delta like 4 Delta like 4 homolog Delta like 4 protein Delta like canonical Notch ligand 4 Delta like protein 4 Delta-like 4 (Drosophila) Delta-like protein 4 Delta4 DLL 4 Dll4 DLL4_HUMAN Drosophila Delta homolog 4 hdelta2 Homeobox protein DLL-4 MGC126344 Notch ligand delta 2 Notch ligand DLL4 Notch ligand DLL4 precursor XDLL-4 |
Images
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Fig1:
Western blot analysis of DLL4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: Mouse placenta tissue lysate |
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Fig2: Flow cytometric analysis of DLL4 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-20, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.