DLL4 Rabbit Polyclonal Antibody
cat.: ER1902-20
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 75 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to N terminal Human DLL4.
Positive control: 293 cell lysate, mouse placenta tissue lysate, 293T.
Subcellular location: Cell membrane, Membrane.
Recommended Dilutions:
  WB
  FC

1:500-1:2,000
1:50-1:100
Uniprot #: SwissProt: Q9NR61 Human | Q9JI71 Mouse
Alternative names: AOS6 Delta 4 delta 4 precursor Delta ligand 4 delta ligand 4 precursor Delta like 4 Delta like 4 homolog Delta like 4 protein Delta like canonical Notch ligand 4 Delta like protein 4 Delta-like 4 (Drosophila) Delta-like protein 4 Delta4 DLL 4 Dll4 DLL4_HUMAN Drosophila Delta homolog 4 hdelta2 Homeobox protein DLL-4 MGC126344 Notch ligand delta 2 Notch ligand DLL4 Notch ligand DLL4 precursor XDLL-4
Images
ER1902-20_1.jpg Fig1: Western blot analysis of DLL4 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-20, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: Mouse placenta tissue lysate
ER1902-20_2.jpg Fig2: Flow cytometric analysis of DLL4 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-20, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.