CD68 Rabbit Polyclonal Antibody
cat.: ER1902-21
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 37 kDa
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal Human CD68.
Positive control: Human spleen tissue lysates, SiHa.
Subcellular location: Cell membrane, endosome membrane, lysosome membrane.
Recommended Dilutions:
  WB
  FC
1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: P34810 Human
Alternative names: CD 68 CD68 CD68 antigen CD68 molecule CD68_HUMAN DKFZp686M18236 gp11 Gp110 LAMP4 Macrophage antigen CD68 (microsialin) MACROPHAGE ANTIGEN CD68 Macrosialin SCARD1 Scavenger receptor class D member 1
Images
ER1902-21_1.jpg Fig1: Western blot analysis of CD68 on human spleen tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-21, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-21_2.jpg Fig2: Flow cytometric analysis of CD68 was done on SiHa cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-21, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.