GSDMC Rabbit Polyclonal Antibody
cat.: ER1902-22
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size 58 kDa.
Isotype: IgG
Immunogen: Recombinant protein within Human GSDMC aa 1-220 / 508.
Positive control: Human stomach tissue lysates, human lung tissue, human kidney tissue, A549.
Subcellular location: Cell membrane, cytosol.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:1,000-1:2,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9BYG8 Human
Alternative names: Gasdermin C Gasdermin-C GSDMC GSDMC_HUMAN Melanoma derived leucine zipper, extra nuclear factor Melanoma-derived leucine zipper-containing extranuclear factor MLZE
Images
ER1902-22_1.jpg Fig1: Western blot analysis of GSDMC on human stomach tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-22, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-22_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-GSDMC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-22_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GSDMC antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-22, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-22_4.jpg Fig4: Flow cytometric analysis of GSDMC was done on A549 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-22, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.