ETN1 Rabbit Polyclonal Antibody
cat.: ER1902-23
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse
Applications: WB, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 50 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human ETN1 aa 19-68 / 456.
Positive control: Human plasma lysates, F9, JAR.
Subcellular location: Plasma membrane.
Recommended Dilutions:
  WB
  ICC
  FC

1:500-1:1,000
1:50-1:100
1:50-1:100
Uniprot #: SwissProt: Q99808 Human | Q9JIM1 Mouse
Alternative names: Equilibrative NBMPR-sensitive nucleoside transporter equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter Equilibrative nucleoside transporter 1 es-type MGC1465 MGC3778 Nucleoside transporter Nucleoside transporter, es-type OTTHUMP00000016506 OTTHUMP00000016507 OTTHUMP00000016508 OTTHUMP00000016509 OTTHUMP00000016510 OTTHUMP00000016511 OTTHUMP00000016512 S29A1_HUMAN Slc29a1 solute carrier family 29 (equilibrative nucleoside transporter), member 1 solute carrier family 29 (nucleoside transporters), member 1 Solute carrier family 29 member 1
Images
ER1902-23_1.jpg Fig1: Western blot analysis of ETN1 on Human plasma lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-23, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-23_2.jpg Fig2: ICC staining of ETN1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-23, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).
ER1902-23_3.jpg Fig3: Flow cytometric analysis of ETN1 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-23, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.