ENT1 Rabbit Polyclonal Antibody
cat.: ER1902-23
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IF-Cell, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human ENT1 aa 19-68 / 456. |
| Positive control: | Human plasma lysate, F9, JAR. |
| Subcellular location: | Plasma membrane. |
| Recommended Dilutions:
WB IF-Cell FC |
1:500-1:1,000 1:50-1:100 1:50-1:100 |
| Uniprot #: | SwissProt: Q99808 Human | Q9JIM1 Mouse |
| Alternative names: | Equilibrative NBMPR-sensitive nucleoside transporter equilibrative nitrobenzylmercaptopurine riboside (NBMPR)-sensitive nucleoside transporter Equilibrative nitrobenzylmercaptopurine riboside-sensitive nucleoside transporter Equilibrative nucleoside transporter 1 es-type MGC1465 MGC3778 Nucleoside transporter Nucleoside transporter, es-type OTTHUMP00000016506 OTTHUMP00000016507 OTTHUMP00000016508 OTTHUMP00000016509 OTTHUMP00000016510 OTTHUMP00000016511 OTTHUMP00000016512 S29A1_HUMAN Slc29a1 solute carrier family 29 (equilibrative nucleoside transporter), member 1 solute carrier family 29 (nucleoside transporters), member 1 Solute carrier family 29 member 1 |
Images
|
Fig1:
Western blot analysis of ENT1 on human plasma lysate with Rabbit anti-ENT1 antibody (ER1902-23) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minute 23 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1902-23, 1/1,000 in 5% NFDM/TBST, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 50 kDa |
|
Fig2: ICC staining of ENT1 in F9 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-23, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue). |
|
Fig3: Flow cytometric analysis of ENT1 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-23, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.