PPAR delta Rabbit Polyclonal Antibody
cat.: ER1902-24
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 50 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within human PPARD aa 260-441. |
| Positive control: | SH-SY5Y cell lysate, NIH/3T3 cell lysate, Mouse colon tissue lysates, SH-SY5Y. |
| Subcellular location: | Nucleus. |
| Recommended Dilutions:
WB FC |
1:500-1:1000 1:50-1:100 |
| Uniprot #: | SwissProt: Q03181 Human | P35396 Mouse |
| Alternative names: | FAAR MGC3931 NR1C2 NUC1 NUCI NUCII Nuclear hormone receptor 1 Nuclear receptor subfamily 1 group C member 2 Peroxisome proliferative activated receptor delta Peroxisome proliferator-activated receptor beta (PPAR-beta) Peroxisome proliferator-activated receptor beta Peroxisome proliferator-activated receptor delta PPAR beta PPAR-beta PPAR-delta PPAR-ß PPARB ppard PPARD_HUMAN |
Images
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Fig1:
Western blot analysis of PPAR delta on different lysates with Rabbit anti-PPAR delta antibody (ER1902-24) at 1/1,000 dilution. Lane 1: SH-SY5Y (Human neuroblastoma cell) cell lysate (10 µg/Lane) Lane 2: NIH/3T3 (Mouse fibroblast) cell lysate (10 µg/Lane) Lane 3: Mouse colon tissue lysate (20 µg/Lane) Exposure time: 60 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1902-24, 1/1,000 in 5% BSA, overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50 kDa Observed band size: 50 kDa |
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Fig2: Flow cytometric analysis of PPAR delta was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-24, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.