GM648 Rabbit Polyclonal Antibody
cat.: ER1902-26
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Mouse
Applications: WB, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 23 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse GM648 aa 1-50 / 213.
Positive control: Mouse placenta tissue lysate, mouse heart tissue lysate, mouse cerebellum tissue lysate, mouse liver tissue, mouse kidney tissue, mouse fallopian tube tissue.
Recommended Dilutions:
  WB
  IHC-P

1:500-1:1,000
1:50-1:200
Uniprot #: SwissProt: Q3UJ22 Mouse
Alternative names: Gene model 648 Predicted gene 648 Gm648
Images
ER1902-26_1.jpg Fig1: Western blot analysis of GM648 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-26, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Mouse placenta tissue lysate
Lane 2: Mouse heart tissue lysate
Lane 3: Mouse cerebellum tissue lysate
ER1902-26_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GM648 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-26, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-26_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-GM648 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-26, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-26_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-GM648 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-26, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.