Transglutaminase 2 Rabbit Polyclonal Antibody
cat.: ER1902-28
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Dog
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 77 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Transglutaminase 2 aa 317-520 / 687.
Positive control: K-562 cell lysate, HeLa cell lysate, Mouse placenta tissue lysate, HeLa, human lung tissue, human placenta tissue, mouse placenta tissue, rat placenta tissue.
Subcellular location: Cell membrane, Chromosome, Cytoplasm, Extracellular matrix, Membrane, Mitochondrion, Nucleus, Secreted.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:1,000
1:1,000-1:5,000
1:100
1:1,000
Uniprot #: SwissProt: P21980 Human | P21981 Mouse
Entrez Gene: 56083 Rat
Alternative names: ALPHA SUBUNIT C polypeptide EC 2.3.2.13 epididymis secretory protein Li 45 G alpha h G[a]h Gh CLASS G ALPHA h GNAH GNAH G PROTEIN H POLYPEPTIDE HEL-S-45 Protein glutamine gamma glutamyltransferase 2 Protein-glutamine gamma-glutamyltransferase 2 TG 2 TG(C) TG2 TGase C TGase H TGase-2 TgaseII TGC TGC GUANINE NUCLEOTIDE BINDING PROTEIN TGM2 TGM2_HUMAN Tissue transglutaminase Transglutaminase 2 Transglutaminase 2 C polypeptide Transglutaminase C Transglutaminase H Transglutaminase-2 tTG tTGas
Images
ER1902-28_1.jpg Fig1: Western blot analysis of Transglutaminase 2 on different lysates with Rabbit anti-Transglutaminase 2 antibody (ER1902-28) at 1/1,000 dilution.

Lane 1: K-562 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Mouse placenta tissue lysate

Lysates/proteins at 20 µg/Lane.

Predicted band size: 77 kDa
Observed band size: 70-77 kDa

Exposure time: 3 seconds; ECL: K1801;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (ER1902-28) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
ER1902-28_2.jpg Fig2: All lanes: Western blot analysis of Transglutaminase 2 with anti-Transglutaminase 2 antibody (ER1902-28) at 1/500 dilution.
Lane 1: Wild-type MDCK whole cell lysate (10 µg).
Lane 2: Transglutaminase 2 knockout MDCK whole cell lysate (10 µg).

ER1902-28 was shown to specifically react with Transglutaminase 2 in wild-type MDCK cells. No band was observed when Transglutaminase 2 knockout sample was tested. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1902-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER1902-28_3.jpg Fig3: Immunocytochemistry analysis of HeLa cells labeling Transglutaminase 2 with Rabbit anti-Transglutaminase 2 antibody (ER1902-28) at 1/100 dilution.

Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Transglutaminase 2 antibody (ER1902-28) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
ER1902-28_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung tissue with Rabbit anti-Transglutaminase 2 antibody (ER1902-28) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-28) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-28_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue with Rabbit anti-Transglutaminase 2 antibody (ER1902-28) at 1/5,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-28) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-28_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse placenta tissue with Rabbit anti-Transglutaminase 2 antibody (ER1902-28) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-28_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded rat placenta tissue with Rabbit anti-Transglutaminase 2 antibody (ER1902-28) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-28) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-28_8.jpg Fig8: Flow cytometric analysis of HeLa cells labeling Transglutaminase 2.

Cells were fixed and permeabilized. Then stained with the primary antibody (ER1902-28, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.