Transglutaminase 2 Rabbit Polyclonal Antibody
cat.: ER1902-28
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat, Dog
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 77 kDa
Isotype: IgG
Immunogen: Recombinant protein within Human Transglutaminase 2 aa 317-520 / 687.
Positive control: HUVEC cell lysate, mouse lung tissue lysate, Wild-type MDCK whole cell lysate, HUVEC, Siha, Rat uterus tissue, Human lung cancer tissue, human placenta tissue, mouse heart tissue, Hela.
Subcellular location: Cell membrane, Chromosome, Cytoplasm, Extracellular matrix, Membrane, Mitochondrion, Nucleus, Secreted.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:1000-1:5000
1:200-1:1000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P21980 Human | P21981 Mouse
Alternative names: ALPHA SUBUNIT C polypeptide EC 2.3.2.13 epididymis secretory protein Li 45 G alpha h G[a]h Gh CLASS G ALPHA h GNAH GNAH G PROTEIN H POLYPEPTIDE HEL-S-45 Protein glutamine gamma glutamyltransferase 2 Protein-glutamine gamma-glutamyltransferase 2 TG 2 TG(C) TG2 TGase C TGase H TGase-2 TgaseII TGC TGC GUANINE NUCLEOTIDE BINDING PROTEIN TGM2 TGM2_HUMAN Tissue transglutaminase Transglutaminase 2 Transglutaminase 2 C polypeptide Transglutaminase C Transglutaminase H Transglutaminase-2 tTG tTGas
Images
ER1902-28_1.jpg Fig1: Western blot analysis of Transglutaminase 2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-28, 1/2,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: HUVEC cell lysate
Lane 2: Mouse lung tissue lysate
ER1902-28_2.jpg Fig2: All lanes: Western blot analysis of Transglutaminase 2 with anti-Transglutaminase 2 antibody (ER1902-28) at 1/500 dilution.
Lane 1: Wild-type MDCK whole cell lysate (10 µg).
Lane 2: Transglutaminase 2 knockout MDCK whole cell lysate (10 µg).

ER1902-28 was shown to specifically react with Transglutaminase 2 in wild-type MDCK cells. No band was observed when Transglutaminase 2 knockout sample was tested. Wild-type and Transglutaminase 2 knockout samples were subjected to SDS-PAGE. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM in TBST for 1 hour at room temperature. The primary antibody (ER1902-28, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG-HRP Secondary Antibody (HA1001) at 1:300,000 dilution was used for 1 hour at room temperature.
ER1902-28_3.jpg Fig3: ICC staining of Transglutaminase 2 in HUVEC cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-28, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-28_4.jpg Fig4: ICC staining of Transglutaminase 2 in Siha cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-28, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-28_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded Rat uterus tissue using anti-Transglutaminase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-28, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-28_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using anti-Transglutaminase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-28, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-28_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Transglutaminase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-28, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-28_8.jpg Fig8: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Transglutaminase 2 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-28, 1/800) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-28_9.jpg Fig9: Flow cytometric analysis of Transglutaminase 2 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-28, 1/100) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; purple).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.