Factor XIIIa Rabbit Polyclonal Antibody
cat.: ER1902-34
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 83 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Factor XIIIa aa 259-463 / 732. |
| Positive control: | Human placenta tissue lysate, human liver tissue lysate, human placenta tissue, Hela. |
| Subcellular location: | Cytoplasm, Secreted. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: P00488 Human |
| Alternative names: | bA525O21.1 (coagulation factor XIII, A1 polypeptide) Coagulation factor XIII A chain Coagulation factor XIII A1 polypeptide Coagulation factor XIII A1 subunit Coagulation factor XIII, A polypeptide Coagulation factor XIIIa F13A F13A_HUMAN F13a1 Factor XIIIA Fibrin stabilizing factor, A subunit Fibrinoligase FSF, A subunit Protein glutamine gamma glutamyltransferase A chain Protein-glutamine gamma-glutamyltransferase A chain TGase Transglutaminase A chain Transglutaminase, plasma Transglutaminase. plasma |
Images
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Fig1:
Western blot analysis of Factor XIIIa on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-34, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: human placenta tissue lysate Lane 2: human liver tissue lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Factor XIIIa antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-34, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of Factor XIIIa was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-34, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.