ER1902-35

CXCL12/SDF1 Rabbit Polyclonal Antibody

cat.: ER1902-35

Product Type:	Rabbit polyclonal IgG, primary antibodies
Species reactivity:	Human, Mouse, Rat
Applications:	WB, IHC-P, IF-Cell, FC
Clonality:	Polyclonal

Form:	Liquid
Storage condition:	Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer:	1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration:	1ug/ul
Purification:	Immunogen affinity purified.
Molecular weight:	Predicted band size: 11 kDa
Isotype:	IgG
Immunogen:	Recombinant protein within huamn SDF1 alpha aa 1-93 / 93.
Positive control:	Recombinant protein lysates, 293T, LOVO, Rat kidney tissue, Human colon cancer tissue, Human kidney tissue, mouse colon tissue, HepG2.
Subcellular location:	Secreted.
Recommended Dilutions: WB IHC-P IF-Cell FC	1:500 1:50-1:600 1:100-1:500 1:50-1:100
Uniprot #:	SwissProt: P48061 Human \| P40224 Mouse \| Q9QZD1 Rat
Alternative names:	12-O-tetradecanoylphorbol 13-acetate repressed protein 1 AI174028 C-X-C motif chemokine 12 Chemokine (C-X-C motif) ligand 12 (stromal cell-derived factor 1) Chemokine (C-X-C motif) ligand 12 Chemokine CXC motif ligand 12 cxcl12 hIRH hSDF-1 Intercrine reduced in hepatomas IRH OTTHUMP00000019491 PBSF Pre-B cell growth-stimulating factor SCYB12 SDF 1 SDF-1 SDF-1-alpha(3-67) SDF-1a SDF-1b SDF1_HUMAN SDF1A SDF1B Stromal cell-derived factor 1 Stromal cell-derived factor 1 delta Stromal cell-derived factor 1 gamma Stromal cell-derived factor 1a Stromal cell-derived factor-1 alpha Thymic lymphoma cell-stimulating factor Tlsf TLSF-a TLSF-b Tlsfa Tlsfb TPAR1

Images

	Fig1: Western blot analysis of CXCL12/SDF1 on recombinant protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-35, 1/20,000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
	Fig2: ICC staining of SDF1 alpha in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-35, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
	Fig3: ICC staining of SDF1 alpha in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-35, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
	Fig4: Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue using anti-SDF1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1902-35, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

	Fig5: Immunohistochemical analysis of paraffin-embedded Human kidney tissue using anti-SDF1 alpha antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1902-35, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig6: Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Rabbit anti-CXCL12/SDF1 antibody (ER1902-35) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1902-35) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig7: Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Rabbit anti-CXCL12/SDF1 antibody (ER1902-35) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH₂O and PBS, and then probed with the primary antibody (ER1902-35) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
	Fig8: Flow cytometric analysis of SDF1 alpha was done on HepG2 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-35, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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