| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Zebrafish |
| Applications: | WB, IF-Tissue, IHC-P |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 58 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within zebrafish SARS2 aa 289-510 / 510. |
| Positive control: | Zebrafish tissue lysate, Zebrafish tissue. |
| Subcellular location: | Mitochondrion. |
| Recommended Dilutions:
WB IF-Tissue IHC-P |
1:200 1:50-1:200 1:50-1:200 |
| Uniprot #: | SwissProt: A3QJX4 Zebrafish |
| Alternative names: | mitochondrial mtSerRS SARS SARS2 SARSM Serine tRNA ligase Serine--tRNA ligase SerRS SerRSmt SERS Seryl tRNA synthetase 2 Seryl-tRNA synthetase Seryl-tRNA(Ser/Sec) synthetase SYS SYSM_HUMAN |
|
Fig1:
Western blot analysis of SARS2 on different lysates with Rabbit anti-SARS2 antibody (ER1902-38) at 1/200 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 1 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1902-38, 1/200 in 5% NFDM/TBST, 2 hours at room temperature Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |
|
Fig2: IF staining of SARS2 in Zebrafish tissue(green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS. |
|
Fig3: Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-SARS2 antibody. Counter stained with hematoxylin. |