ACE2 Rabbit Polyclonal Antibody
cat.: ER1902-40
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 92 kDa |
| Isotype: | IgG |
| Immunogen: | ACE2 transfected 293 cells. |
| Positive control: | Human kidney tissue lysates, human kidney tissue, Vero cell. |
| Subcellular location: | Secreted, Cell membrane. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50 |
| Uniprot #: | SwissProt: Q9BYF1 Human |
| Alternative names: | ACE 2 ACE related carboxypeptidase ACE-related carboxypeptidase ACE2 ACE2_HUMAN ACEH Angiotensin converting enzyme 2 Angiotensin converting enzyme homolog Angiotensin converting enzyme like protein Angiotensin I Converting Enzyme (peptidyl dipeptidase A) 2 Angiotensin I converting enzyme 2 Angiotensin-converting enzyme homolog DKFZP434A014 EC 3.4.17 metalloprotease MPROT 15 Metalloprotease MPROT15 OTTHUMP00000022963 |
Images
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Fig1: Western blot analysis of ACE2 on human kidney tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-40, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. |
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Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-40, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Flow cytometric analysis of ACE2 was done on Vero cells. The cells were stained with the primary antibody (ER1902-40, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1,000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; green). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.