KCNN2 Rabbit Polyclonal Antibody
cat.: ER1902-41
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 64 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within rat KCNN2 aa 531-580 / 580.
Positive control: Rat brain tissue lysates, rat brain tissue, rat cerebellar tissue, mouse brain tissue, rat cerebellar tissue, human colon tissue, F9.
Subcellular location: Membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:2000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9H2S1 Human | P58390 Mouse | P70604 Rat
Alternative names: Apamin sensitive small conductance Ca2+ activated potassium Apamin sensitive small conductance Ca2+ activated potassium channel hSK2 KCa2.2 Kcnn2 KCNN2_HUMAN Potassium intermediate/small conductance calcium activated Potassium intermediate/small conductance calcium activated channel subfamily N member 2 SK2 SKCa 2 SKCa2 Small conductance calcium activated potassium channel Small conductance calcium activated potassium channel protein 2 Small conductance calcium-activated potassium channel protein 2
Images
ER1902-41_1.jpg Fig1: Western blot analysis of KCNN2 on rat brain tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-41, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-41_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-KCNN2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-41, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-41_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rat cerebellar tissue using anti-KCNN2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-41, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-41_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-KCNN2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-41, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-41_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded rat cerebellar tissue using anti-KCNN2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-41, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-41_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-KCNN2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-41, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-41_7.jpg Fig7: Flow cytometric analysis of KCNN2 was done on F9 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-41, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.