Anti-Flavin-containing monooxygenase antibody
cat.: ER1902-44
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Rice
Applications: WB, IF, IHC-P
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/mL.
Purification: Peptide affinity purified.
Molecular weight: 46 kDa
Isotype: IgG
Immunogen: Synthetic peptide within rice Flavin-containing monooxygenase aa 1-30.
Positive control: Rice tissue.
Subcellular location: Integral component of membrane.
Recommended Dilutions:
  WB
  IF
  IHC-P

1:500-1:2,000
1:50-1:100
1:50-1:200
Uniprot #: SwissProt: A2XPX7 Rice
Alternative names: Flavin-containing monooxygenase OsI_14691
Images
ER1902-44_1.jpg Fig1: Western blot analysis of Flavin-containing monooxygenase on rice tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-44, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-44_2.jpg Fig2: Immunofluorescence staining of paraffin- embedded rice tissue using anti-Rubisco activase rabbit polyclonal antibody.The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.(sodium citrate buffer (pH6) for 20 mins.) The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with ER1902-44 at 1/50 dilution for 10 hours at 4℃ and detected using Alexa Fluor® 488 conjugate-Goat anti-Rabbit IgG (H+L) Secondary Antibody at a dilution of 1:500 for 1 hour at room temperature.
ER1902-44_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded rice tissue using anti-Flavin-containing monooxygenase antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-44, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.