Anti-CLIC4 antibody
cat.: ER1902-48
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Peptide affinity purified.
Molecular weight: 29 kDa
Isotype: IgG
Immunogen: Synthetic peptide corresponding to N-terminal Human CLIC4.
Positive control: Rat kidney and mouse kidney tissue lysates, rat skeletal muscle tissue, human kidney tissue, human placenta tissue, mouse heart tissue, 293T.
Subcellular location: Cell junction, Cell membrane, Cytoplasm, Cytoplasmic vesicle, Cytoskeleton, Membrane, Mitochondrion, Nucleus.
Recommended Dilutions:
  WB
  IHC-P
  FC

1:500-1:1000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9Y696 Human | Q9QYB1 Mouse | Q9Z0W7 Rat
Alternative names: Chloride intracellular channel 4 antibody Chloride intracellular channel 4 (mitochondrial) antibody Chloride intracellular channel 4 like antibody Chloride intracellular channel protein 4 antibody Clic4 antibody CLIC4_HUMAN antibody CLIC4L antibody DKFZP566G223 antibody FLJ38640 antibody H1 antibody HUH1 antibody Intracellular chloride ion channel protein p64H1 antibody MC3S5 antibody mtCLIC antibody p64H1 antibody
Images
ER1902-48_1.jpg Fig1: Western blot analysis of CLIC4 on rat kidney (1) and mouse kidney (2) tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-48, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-48_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-CLIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-48, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-48_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CLIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-48, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-48_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-CLIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-48, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-48_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CLIC4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-48, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-48_6.jpg Fig6: Flow cytometric analysis of CLIC4 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-48, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.