Kv1.4 Rabbit Polyclonal Antibody
cat.: ER1902-49
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, ICC, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1 mg/ml.
Purification: Immunogen affinity purified.
Molecular weight: 73 kDa
Isotype: IgG
Immunogen: Synthetic peptide within mouse KCNA4 aa 330-379 / 654.
Positive control: Rat brain tissuet lysates, rat cerebellar tissue, MCF-7, PANC-1, mouse brain.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  IHC-P
  FC

1:500
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P22459 Human | Q61423 Mouse | P15385 Rat
Alternative names: Voltage gated K+ channel HuKII Fetal skeletal muscle potassium channel HBK4 HK1 HPCN2 HUKII Kcna4 KCNA4_HUMAN KCNA4L KCNA8 kv1.4 PCN2 Potassium channel 2 Potassium voltage gated channel shaker related subfamily member 4 Potassium voltage gated channel subfamily A member 4 Potassium voltage-gated channel subfamily A member 4 Rapidly inactivating potassium channel Shaker related potassium channel Kv1.4 Type A potassium channel Voltage gated potassium channel HBK4 Voltage gated potassium channel HK1 Voltage gated potassium channel subunit Kv1.4 Voltage-gated K(+) channel HuKII Voltage-gated potassium channel HBK4 Voltage-gated potassium channel HK1 Voltage-gated potassium channel subunit Kv1.4
Images
ER1902-49_1.jpg Fig1: Western blot analysis of Kv1.4 on rat brain tissuet lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-49, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-49_2.jpg Fig2: ICC staining of Kv1.4 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-49, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).7
ER1902-49_3.jpg Fig3: ICC staining of Kv1.4 in PANC-1 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-49, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-49_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded rat cerebellar tissue using anti-Kv1.4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-49, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-49_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Kv1.4 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-49, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-49_6.jpg Fig6: Flow cytometric analysis of Kv1.4 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-49, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.