NHE-1 Rabbit Polyclonal Antibody
cat.: ER1902-51
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 91/61 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within mouse NHE-1 aa 36-85 / 820.
Positive control: Rat skin lysates, A431, 293T, LOVO, human kidney tissue, mouse colon tissue, human small intestine tissue.
Subcellular location: Cell membrane, Endoplasmic reticulum, Membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:500-1:1000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P19634 Human | Q61165 Mouse | P26431 Rat
Alternative names: amiloride-sensitive APNH APNH1 FLJ42224 Na Li countertransporter Na(+)/H(+) antiporter Na(+)/H(+) exchanger 1 Na+ H+ antiporter amiloride-sensitive Na+ H+ antiporter Na+ H+ exchanger 1 NHE-1 NHE1 OTTHUMP00000004468 SL9A1_HUMAN SLC9A1 Sodium / Hydrogen Exchanger 1 Sodium hydrogen exchanger 1 Sodium/hydrogen exchanger 1 solute carrier family 9 Solute carrier family 9 member 1 Solute carrier family 9 sodium hydrogen exchanger isoform 1 antiporter Na+ H+ amiloride sensitive Solute carrier family 9 subfamily A (NHE1 cation proton antiporter 1) member 1 Solute carrier family 9 subfamily A member 1
Images
ER1902-51_1.jpg Fig1: Western blot analysis of NHE-1 on rat skin lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-51, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-51_2.jpg Fig2: ICC staining of NHE-1 in A431 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-51, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-51_3.jpg Fig3: ICC staining of NHE-1 in 293T cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-51, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-51_4.jpg Fig4: ICC staining of NHE-1 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-51, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-51_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NHE-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-51_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-NHE-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-51, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-51_7.jpg Fig7: Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-NHE-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-51, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-51_8.jpg Fig8: Flow cytometric analysis of NHE-1 was done on 293T cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-51, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.