Product Type: | Rabbit polyclonal IgG, primary antibodies |
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Species reactivity: | Human, Mouse, Rat |
Applications: | WB, FC |
Clonality: | Polyclonal |
Form: | Liquid |
Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
Concentration: | 1ug/ul |
Purification: | Immunogen affinity purified. |
Molecular weight: | 100 kDa |
Isotype: | IgG |
Immunogen: | Synthetic peptide within Human LGR5 / GPR49 aa 499-548 / 907. |
Positive control: | HCT116. |
Subcellular location: | Cell membrane, Golgi apparatus. |
Recommended Dilutions:
WB FC |
1:500-1:1,000 1:50-1:100 |
Uniprot #: | SwissProt: O75473 Human | Q9Z1P4 Mouse Entrez Gene: 299802 Rat |
Alternative names: | FEX G protein coupled receptor 49 G protein coupled receptor 67 g protein-coupled receptor fex G-protein coupled receptor 49 G-protein coupled receptor 67 G-protein coupled receptor HG38 GPCR GPR49 GPR 49 GPR 67 GPR49 GPR67 GRP 49 GRP49 HG 38 HG38 Leucine rich repeat containing G protein coupled receptor 5 Leucine-rich repeat-containing G-protein coupled receptor 5 LGR 5 LGR5 LGR5_HUMAN MGC117008 Orphan G protein coupled receptor HG38 |
Fig1:
Western blot analysis of LGR5/GPR49 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-52, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: 293 cell lysate Lane 2: NIH/3T3 cell lysate |
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Fig2: Flow cytometric analysis of LGR5/GPR49 was done on HCT116 cells. The cells were fixed, permeabilized and stained with LGR5/GPR49 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes. |