LGR5/GPR49 Rabbit Polyclonal Antibody
cat.: ER1902-52
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: 100 kDa
Isotype: IgG
Immunogen: Synthetic peptide within Human LGR5 / GPR49 aa 499-548 / 907.
Positive control: HCT116.
Subcellular location: Cell membrane, Golgi apparatus.
Recommended Dilutions:
  WB
  FC

1:500-1:1,000
1:50-1:100
Uniprot #: SwissProt: O75473 Human | Q9Z1P4 Mouse
Entrez Gene: 299802 Rat
Alternative names: FEX G protein coupled receptor 49 G protein coupled receptor 67 g protein-coupled receptor fex G-protein coupled receptor 49 G-protein coupled receptor 67 G-protein coupled receptor HG38 GPCR GPR49 GPR 49 GPR 67 GPR49 GPR67 GRP 49 GRP49 HG 38 HG38 Leucine rich repeat containing G protein coupled receptor 5 Leucine-rich repeat-containing G-protein coupled receptor 5 LGR 5 LGR5 LGR5_HUMAN MGC117008 Orphan G protein coupled receptor HG38
Images
ER1902-52_1.jpg Fig1: Western blot analysis of LGR5/GPR49 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-52, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: 293 cell lysate
Lane 2: NIH/3T3 cell lysate
ER1902-52_2.jpg Fig2: Flow cytometric analysis of LGR5/GPR49 was done on HCT116 cells. The cells were fixed, permeabilized and stained with LGR5/GPR49 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). After incubation of the primary antibody on room temperature for an hour, the cells was stained with a Alexa Fluor™ 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.