| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 100 kDa |
| Isotype: | IgG |
| Immunogen: | Synthetic peptide within Human LGR5 aa 499-548 / 907. |
| Positive control: | HEK-293 (Human embryonic kidney cell) cell lysate. |
| Subcellular location: | Cell membrane, Golgi apparatus. |
| Recommended Dilutions:
WB |
1:500-1:1,000 |
| Uniprot #: | SwissProt: O75473 Human |
| Alternative names: | FEX G protein coupled receptor 49 G protein coupled receptor 67 g protein-coupled receptor fex G-protein coupled receptor 49 G-protein coupled receptor 67 G-protein coupled receptor HG38 GPCR GPR49 GPR 49 GPR 67 GPR49 GPR67 GRP 49 GRP49 HG 38 HG38 Leucine rich repeat containing G protein coupled receptor 5 Leucine-rich repeat-containing G-protein coupled receptor 5 LGR 5 LGR5 LGR5_HUMAN MGC117008 Orphan G protein coupled receptor HG38 |
|
Fig1:
Western blot analysis of LGR5 on HEK-293 (Human embryonic kidney cell) cell lysate with Rabbit anti-LGR5 antibody (ER1902-52) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Exposure time: 3 minutes; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1902-52, 1/1,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature |