Perilipin-5 Rabbit Polyclonal Antibody
cat.: ER1902-53
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human, Mouse |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | 51 kDa |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Perilipin-5 aa 80-302 / 463. |
| Positive control: | Human liver tissue lysate, human marrow tissue lysate, mouse heart tissue, JAR. |
| Subcellular location: | Cytoplasm, Lipid droplet, Mitochondrion. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q00G26 Human | Q8BVZ1 Mouse |
| Alternative names: | Lipid storage droplet protein 5 LSDA5 MLDP OXPAT Perilipin 5 Perilipin-5 PLIN5 PLIN5_HUMAN |
Images
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Fig1: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Perilipin-5 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-53, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig2: Flow cytometric analysis of Perilipin-5 was done on JAR cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-53, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black). |
|
Fig3:
Western blot analysis of Perilipin-5 on Mouse liver tissue lysates with Rabbit anti-Perilipin-5 antibody (ER1902-53) at 1/5,000 dilution. Lysates/proteins at 15 µg/Lane. Exposure time: 6 seconds; ECL: K1801 Blocking: 5% NFDM/TBST, 1 hour at room temperature Primary antibody: ER1902-53, 1/5,000 in primary antibody dilution buffer (K1803), overnight at 4 ℃ Secondary antibody: Goat anti-Rabbit IgG-HRP (HA1001), 1/50,000 in 5% NFDM/TBST, 1 hour at room temperature Predicted band size: 50.8 kDa Observed band size: 50.8 kDa |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.