CEACAM6 Rabbit Polyclonal Antibody
cat.: ER1902-54
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, IF-Cell, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 37 kDa.
Isotype: IgG
Immunogen: Recombinant protein within Human CEACAM6 aa 49-224 / 344.
Positive control: JAR cell lysate, rat lung tissue lysate, MCF-7 cell lysate, human lung tissue lysate, LOVO, MCF-7, human lung cancer tissue, human colon cancer tissue, human appendix cancer tissue.
Subcellular location: Cell membrane, Membrane.
Recommended Dilutions:
  WB
  IHC-P
  IF-Cell
  FC
1:500-1:1,000
1:50-1:200
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: P40199 Human
Alternative names: Carcinoembryonic antigen related cell adhesion molecule 6 Carcinoembryonic antigen related cell adhesion molecule 6 (non specific cross reacting antigen) Carcinoembryonic antigen-related cell adhesion molecule 6 CD 66c CD66c CD66c antigen CEA LIKE PROTEIN CEACAM 6 CEACAM6 CEAL CEAM6_HUMAN MGC93832 NCA Non specific cross reacting antigen Non-specific crossreacting antigen Normal cross reacting antigen Normal cross-reacting antigen
Images
ER1902-54_1.jpg Fig1: Western blot analysis of CEACAM6 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-54, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: JAR cell lysate
Lane 2: Rat lung tissue lysate
Lane 3: MCF-7 cell lysate
Lane 2: Human lung tissue lysate
ER1902-54_2.jpg Fig2: ICC staining of CEACAM6 in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-54, 1/200) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-54_3.jpg Fig3: ICC staining of CEACAM6 in MCF-7 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (ER1902-54, 1/100) for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
ER1902-54_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CEACAM6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-54, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-54_5.jpg Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CEACAM6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-54, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-54_6.jpg Fig6: Immunohistochemical analysis of paraffin-embedded human appendix cancer tissue using anti-CEACAM6 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-54, 1/100) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-54_7.jpg Fig7: Flow cytometric analysis of CEACAM6 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-54, 1/100) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.