Drebrin Rabbit Polyclonal Antibody
cat.: ER1902-55
| Product Type: | Rabbit polyclonal IgG, primary antibodies |
|---|---|
| Species reactivity: | Human |
| Applications: | WB, IHC-P, FC |
| Clonality: | Polyclonal |
| Form: | Liquid |
| Storage condition: | Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles. |
| Storage buffer: | 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide. |
| Concentration: | 1ug/ul |
| Purification: | Immunogen affinity purified. |
| Molecular weight: | Predicted band size: 71 kDa. |
| Isotype: | IgG |
| Immunogen: | Recombinant protein within Human Drebrin aa 275-468 / 649. |
| Positive control: | A549 cell lysate, PC-3M cell lysate, HepG2 cell lysate, 293 cell lysate, Siha cell lysate, human lung cancer tissue, human kidney tissue, SHSY5Y. |
| Subcellular location: | Cell junction, Cell projection, Cytoplasm. |
| Recommended Dilutions:
WB IHC-P FC |
1:500-1:1,000 1:50-1:200 1:50-1:100 |
| Uniprot #: | SwissProt: Q16643 Human |
| Alternative names: | D0S117E DBN 1 DBN1 Developmentally regulated brain protein Developmentally-regulated brain protein DKFZp434D064 DREB_HUMAN Drebrin 1 Drebrin Drebrin E Drebrin E2 Drebrin1 DrebrinE |
Images
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Fig1:
Western blot analysis of Drebrin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-55, 1/1000) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature. Positive control: Lane 1: A549 cell lysate Lane 2: PC-3M cell lysate Lane 3: HepG2 cell lysate Lane 4: 293 cell lysate Lane 5: Siha cell lysate |
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Fig2: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Drebrin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-55, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Drebrin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-55, 1/200) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. |
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Fig4: Flow cytometric analysis of Drebrin was done on SHSY5Y cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-55, 1/100) (yellow). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-rabbit IgG Secondary antibody at 1/500 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; purple). |
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.