P2X2 Rabbit Polyclonal Antibody
cat.: ER1902-59
Product Type: Rabbit polyclonal IgG, primary antibodies
Species reactivity: Human, Mouse, Rat
Applications: WB, IHC-P, FC
Clonality: Polyclonal
Form: Liquid
Storage condition: Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Storage buffer: 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration: 1ug/ul
Purification: Immunogen affinity purified.
Molecular weight: Predicted band size: 53 kDa.
Isotype: IgG
Immunogen: Synthetic peptide within C-terminal mouse P2X2.
Positive control: Mouse brain tissue lysates, rat brain tissue, human esophagus tissue, mouse brain tissue, MCF-7.
Subcellular location: Cell membrane.
Recommended Dilutions:
  WB
  IHC-P
  FC
1:500-1:1,000
1:50-1:200
1:50-1:100
Uniprot #: SwissProt: Q9UBL9 Human | Q8K3P1 Mouse | P49653 Rat
Alternative names: ATP receptor MGC129601 P2RX 2 P2RX2 P2RX2_HUMAN P2X purinoceptor 2 P2X Receptor subunit 2 P2X2 P2X2a Purinergic receptor Purinergic receptor P2X ligand gated ion channel 2 Purinergic receptor P2X2 isoform A Purinergic receptor P2X2 isoform B Purinergic receptor P2X2 isoform C Purinergic receptor P2X2 isoform D Purinergic receptor P2X2 isoform H Purinergic receptor P2X2 isoform I
Images
ER1902-59_1.jpg Fig1: Western blot analysis of P2X2 on mouse brain tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-59, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
ER1902-59_2.jpg Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-P2X2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-59_3.jpg Fig3: Immunohistochemical analysis of paraffin-embedded human esophagus tissue using anti-P2X2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-59_4.jpg Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-P2X2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (ER1902-59, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
ER1902-59_5.jpg Fig5: Flow cytometric analysis of P2X2 was done on MCF-7 cells. The cells were fixed, permeabilized and stained with the primary antibody (ER1902-59, 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Note: All products are “FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE”.